Expressing:Article Title: Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype.
Article Snippet: Cat# NC15-Research Chemicals, peptides, and recombinant proteins Fetal Bovine Serum Biosera Cat# 60923 Penicillin streptomycin solution MilliporeSIgma Cat# P0781 L-glutamine MilliporeSigma Cat# G7513 RPMI 1640 Medium MilliporeSigma Cat# R0993 Viability dye FVS780 BD Biosciences Cat# 565388 Streptavidin-PE Invitrogen Cat# S866 Streptavidin-APC eBioscience Cat# 405207 Trizma hydrochloride solution (Tris-HCl) Sigma-Aldrich Cat# T3038 (Continued on next page) e1 Cell 187, 1–17.e1–e10, September 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAsin Promega Cat# N2515 BshTI ThermoFisher Scientific Cat# FD1464 SalI ThermoFisher Scientific Cat# FD0644 XhoI ThermoFisher Scientific Cat# FD0694 Pfl23II ThermoFisher Scientific Cat# FD0854 LB agar plates with Amp-50 Teknova Cat# L1150 Dimethyl sulfoxide Sigma-Aldrich Cat# 20139 EX-CELL 420 medium Sigma-Aldrich Cat# 14420C Expi293 Expression medium ThermoFisher Scientific Cat# A1435101 Opti-MEM reduced serum medium ThermoFisher Scientific Cat# 31985070 Protein A Sepharose Fast Flow MilliporeSigma Cat# P3296 Phosphate buffered saline tablets Sigma-Aldrich Cat# P4417 CaptureSelect affinity C-Tag XL Affinity Matrix ThermoFisher Scientific Cat# 2943072010 Tween 20 Sigma-Aldrich Cat# P1379 Blocker Casein (thermos Fisher Scientific) ThermoFisher Scientific Cat# 37528 SIGMAFAST p-Nitrophenyl phosphate alkaline phosphatase substrate ThermoFisher Scientific Cat# 37620 Diethanolamine ThermoFisher Scientific Cat# 34064 Pierce Streptavidin coated plates ThermoFisher Scientific Cat# 15124 62 Biotinylated 20-mer PfRH5FL peptides Synthesised by Mimotopes, obtained from Simon J. Draper, Oxford University; Payne et al.18 N/A Ethanolamine (pH 8.5) Carterra Cat# 3626 Glycine (pH 2.0) Carterra Cat# 3640 Sodium acetate (pH 4.5) Carterra Cat# 3623 MES Carterra Cat# 3625 HEPES Sigma-Aldrich Cat# H3375 Hypoxanthine Sigma-Aldrich Cat# H9377 HiTrap Protein G column Cytiva Cat# 17040501 LambdaFabSelect column Cytiva Cat# 17548211 RH5.1 Simon J. Draper, University of Oxford; Jin et al.16 N/A PfRH5DNC-Biotin This paper N/A PfCyRPA Simon J. Draper, University of Oxford; Alanine et al.26 N/A PfRH5DNL Simon J. Draper, University of Oxford; Wright et al.20 N/A Basigin Simon J. Draper, University of Oxford; Wright et al.20 N/A Critical commercial assays Amine Coupling Kit Sulfo-N-hydroxysuccinimide Cytiva Cat# BR1000-50 Human Pan-B cell enrichment kit StemCell Technologies Cat# 19554 ExpiFectamine 293 Transfection Kit ThermoFisher Scientific Cat# A14525 PCR purification kit Qiagen Cat# 28006 QIAprep spin miniprep kit Qiagen Cat# 27104 Deposited data Crystal structure of RH5DNL:R5.251 complex This paper PDB: 8QKR Crystal structure of RH5DNL:R5.034 complex This paper PDB: 8QKS Experimental models: Cell lines Human HEK293F ThermoFisher Scientific Cat# A14527 (Continued on next page) ll OPEN ACCESS Cell 187, 1–17.e1–e10, September 5, 2024 e2 Please cite this article in press as: Barrett et al., Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.015 Article .. Long, NIAID N/A P. falciparum NF54 Brandon K. Wilder, OHSU N/A Liver-humanized FRGN KO mice Yecuris N/A Oligonucleotides Primers for Vg, Vk, and Vl antibody heavy and light chain sequence reverse-transcription and nested PCR Hedda Wardemann; Tiller et al.48 N/A Primers for amplifying and cloning human Fab fragment sequences.
Saline:Article Title: Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype.
Article Snippet: Cat# NC15-Research Chemicals, peptides, and recombinant proteins Fetal Bovine Serum Biosera Cat# 60923 Penicillin streptomycin solution MilliporeSIgma Cat# P0781 L-glutamine MilliporeSigma Cat# G7513 RPMI 1640 Medium MilliporeSigma Cat# R0993 Viability dye FVS780 BD Biosciences Cat# 565388 Streptavidin-PE Invitrogen Cat# S866 Streptavidin-APC eBioscience Cat# 405207 Trizma hydrochloride solution (Tris-HCl) Sigma-Aldrich Cat# T3038 (Continued on next page) e1 Cell 187, 1–17.e1–e10, September 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAsin Promega Cat# N2515 BshTI ThermoFisher Scientific Cat# FD1464 SalI ThermoFisher Scientific Cat# FD0644 XhoI ThermoFisher Scientific Cat# FD0694 Pfl23II ThermoFisher Scientific Cat# FD0854 LB agar plates with Amp-50 Teknova Cat# L1150 Dimethyl sulfoxide Sigma-Aldrich Cat# 20139 EX-CELL 420 medium Sigma-Aldrich Cat# 14420C Expi293 Expression medium ThermoFisher Scientific Cat# A1435101 Opti-MEM reduced serum medium ThermoFisher Scientific Cat# 31985070 Protein A Sepharose Fast Flow MilliporeSigma Cat# P3296 Phosphate buffered saline tablets Sigma-Aldrich Cat# P4417 CaptureSelect affinity C-Tag XL Affinity Matrix ThermoFisher Scientific Cat# 2943072010 Tween 20 Sigma-Aldrich Cat# P1379 Blocker Casein (thermos Fisher Scientific) ThermoFisher Scientific Cat# 37528 SIGMAFAST p-Nitrophenyl phosphate alkaline phosphatase substrate ThermoFisher Scientific Cat# 37620 Diethanolamine ThermoFisher Scientific Cat# 34064 Pierce Streptavidin coated plates ThermoFisher Scientific Cat# 15124 62 Biotinylated 20-mer PfRH5FL peptides Synthesised by Mimotopes, obtained from Simon J. Draper, Oxford University; Payne et al.18 N/A Ethanolamine (pH 8.5) Carterra Cat# 3626 Glycine (pH 2.0) Carterra Cat# 3640 Sodium acetate (pH 4.5) Carterra Cat# 3623 MES Carterra Cat# 3625 HEPES Sigma-Aldrich Cat# H3375 Hypoxanthine Sigma-Aldrich Cat# H9377 HiTrap Protein G column Cytiva Cat# 17040501 LambdaFabSelect column Cytiva Cat# 17548211 RH5.1 Simon J. Draper, University of Oxford; Jin et al.16 N/A PfRH5DNC-Biotin This paper N/A PfCyRPA Simon J. Draper, University of Oxford; Alanine et al.26 N/A PfRH5DNL Simon J. Draper, University of Oxford; Wright et al.20 N/A Basigin Simon J. Draper, University of Oxford; Wright et al.20 N/A Critical commercial assays Amine Coupling Kit Sulfo-N-hydroxysuccinimide Cytiva Cat# BR1000-50 Human Pan-B cell enrichment kit StemCell Technologies Cat# 19554 ExpiFectamine 293 Transfection Kit ThermoFisher Scientific Cat# A14525 PCR purification kit Qiagen Cat# 28006 QIAprep spin miniprep kit Qiagen Cat# 27104 Deposited data Crystal structure of RH5DNL:R5.251 complex This paper PDB: 8QKR Crystal structure of RH5DNL:R5.034 complex This paper PDB: 8QKS Experimental models: Cell lines Human HEK293F ThermoFisher Scientific Cat# A14527 (Continued on next page) ll OPEN ACCESS Cell 187, 1–17.e1–e10, September 5, 2024 e2 Please cite this article in press as: Barrett et al., Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.015 Article .. Long, NIAID N/A P. falciparum NF54 Brandon K. Wilder, OHSU N/A Liver-humanized FRGN KO mice Yecuris N/A Oligonucleotides Primers for Vg, Vk, and Vl antibody heavy and light chain sequence reverse-transcription and nested PCR Hedda Wardemann; Tiller et al.48 N/A Primers for amplifying and cloning human Fab fragment sequences.
Transfection:Article Title: Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype.
Article Snippet: Cat# NC15-Research Chemicals, peptides, and recombinant proteins Fetal Bovine Serum Biosera Cat# 60923 Penicillin streptomycin solution MilliporeSIgma Cat# P0781 L-glutamine MilliporeSigma Cat# G7513 RPMI 1640 Medium MilliporeSigma Cat# R0993 Viability dye FVS780 BD Biosciences Cat# 565388 Streptavidin-PE Invitrogen Cat# S866 Streptavidin-APC eBioscience Cat# 405207 Trizma hydrochloride solution (Tris-HCl) Sigma-Aldrich Cat# T3038 (Continued on next page) e1 Cell 187, 1–17.e1–e10, September 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAsin Promega Cat# N2515 BshTI ThermoFisher Scientific Cat# FD1464 SalI ThermoFisher Scientific Cat# FD0644 XhoI ThermoFisher Scientific Cat# FD0694 Pfl23II ThermoFisher Scientific Cat# FD0854 LB agar plates with Amp-50 Teknova Cat# L1150 Dimethyl sulfoxide Sigma-Aldrich Cat# 20139 EX-CELL 420 medium Sigma-Aldrich Cat# 14420C Expi293 Expression medium ThermoFisher Scientific Cat# A1435101 Opti-MEM reduced serum medium ThermoFisher Scientific Cat# 31985070 Protein A Sepharose Fast Flow MilliporeSigma Cat# P3296 Phosphate buffered saline tablets Sigma-Aldrich Cat# P4417 CaptureSelect affinity C-Tag XL Affinity Matrix ThermoFisher Scientific Cat# 2943072010 Tween 20 Sigma-Aldrich Cat# P1379 Blocker Casein (thermos Fisher Scientific) ThermoFisher Scientific Cat# 37528 SIGMAFAST p-Nitrophenyl phosphate alkaline phosphatase substrate ThermoFisher Scientific Cat# 37620 Diethanolamine ThermoFisher Scientific Cat# 34064 Pierce Streptavidin coated plates ThermoFisher Scientific Cat# 15124 62 Biotinylated 20-mer PfRH5FL peptides Synthesised by Mimotopes, obtained from Simon J. Draper, Oxford University; Payne et al.18 N/A Ethanolamine (pH 8.5) Carterra Cat# 3626 Glycine (pH 2.0) Carterra Cat# 3640 Sodium acetate (pH 4.5) Carterra Cat# 3623 MES Carterra Cat# 3625 HEPES Sigma-Aldrich Cat# H3375 Hypoxanthine Sigma-Aldrich Cat# H9377 HiTrap Protein G column Cytiva Cat# 17040501 LambdaFabSelect column Cytiva Cat# 17548211 RH5.1 Simon J. Draper, University of Oxford; Jin et al.16 N/A PfRH5DNC-Biotin This paper N/A PfCyRPA Simon J. Draper, University of Oxford; Alanine et al.26 N/A PfRH5DNL Simon J. Draper, University of Oxford; Wright et al.20 N/A Basigin Simon J. Draper, University of Oxford; Wright et al.20 N/A Critical commercial assays Amine Coupling Kit Sulfo-N-hydroxysuccinimide Cytiva Cat# BR1000-50 Human Pan-B cell enrichment kit StemCell Technologies Cat# 19554 ExpiFectamine 293 Transfection Kit ThermoFisher Scientific Cat# A14525 PCR purification kit Qiagen Cat# 28006 QIAprep spin miniprep kit Qiagen Cat# 27104 Deposited data Crystal structure of RH5DNL:R5.251 complex This paper PDB: 8QKR Crystal structure of RH5DNL:R5.034 complex This paper PDB: 8QKS Experimental models: Cell lines Human HEK293F ThermoFisher Scientific Cat# A14527 (Continued on next page) ll OPEN ACCESS Cell 187, 1–17.e1–e10, September 5, 2024 e2 Please cite this article in press as: Barrett et al., Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.015 Article .. Long, NIAID N/A P. falciparum NF54 Brandon K. Wilder, OHSU N/A Liver-humanized FRGN KO mice Yecuris N/A Oligonucleotides Primers for Vg, Vk, and Vl antibody heavy and light chain sequence reverse-transcription and nested PCR Hedda Wardemann; Tiller et al.48 N/A Primers for amplifying and cloning human Fab fragment sequences.
Polymerase Chain Reaction:Article Title: Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype.
Article Snippet: Cat# NC15-Research Chemicals, peptides, and recombinant proteins Fetal Bovine Serum Biosera Cat# 60923 Penicillin streptomycin solution MilliporeSIgma Cat# P0781 L-glutamine MilliporeSigma Cat# G7513 RPMI 1640 Medium MilliporeSigma Cat# R0993 Viability dye FVS780 BD Biosciences Cat# 565388 Streptavidin-PE Invitrogen Cat# S866 Streptavidin-APC eBioscience Cat# 405207 Trizma hydrochloride solution (Tris-HCl) Sigma-Aldrich Cat# T3038 (Continued on next page) e1 Cell 187, 1–17.e1–e10, September 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAsin Promega Cat# N2515 BshTI ThermoFisher Scientific Cat# FD1464 SalI ThermoFisher Scientific Cat# FD0644 XhoI ThermoFisher Scientific Cat# FD0694 Pfl23II ThermoFisher Scientific Cat# FD0854 LB agar plates with Amp-50 Teknova Cat# L1150 Dimethyl sulfoxide Sigma-Aldrich Cat# 20139 EX-CELL 420 medium Sigma-Aldrich Cat# 14420C Expi293 Expression medium ThermoFisher Scientific Cat# A1435101 Opti-MEM reduced serum medium ThermoFisher Scientific Cat# 31985070 Protein A Sepharose Fast Flow MilliporeSigma Cat# P3296 Phosphate buffered saline tablets Sigma-Aldrich Cat# P4417 CaptureSelect affinity C-Tag XL Affinity Matrix ThermoFisher Scientific Cat# 2943072010 Tween 20 Sigma-Aldrich Cat# P1379 Blocker Casein (thermos Fisher Scientific) ThermoFisher Scientific Cat# 37528 SIGMAFAST p-Nitrophenyl phosphate alkaline phosphatase substrate ThermoFisher Scientific Cat# 37620 Diethanolamine ThermoFisher Scientific Cat# 34064 Pierce Streptavidin coated plates ThermoFisher Scientific Cat# 15124 62 Biotinylated 20-mer PfRH5FL peptides Synthesised by Mimotopes, obtained from Simon J. Draper, Oxford University; Payne et al.18 N/A Ethanolamine (pH 8.5) Carterra Cat# 3626 Glycine (pH 2.0) Carterra Cat# 3640 Sodium acetate (pH 4.5) Carterra Cat# 3623 MES Carterra Cat# 3625 HEPES Sigma-Aldrich Cat# H3375 Hypoxanthine Sigma-Aldrich Cat# H9377 HiTrap Protein G column Cytiva Cat# 17040501 LambdaFabSelect column Cytiva Cat# 17548211 RH5.1 Simon J. Draper, University of Oxford; Jin et al.16 N/A PfRH5DNC-Biotin This paper N/A PfCyRPA Simon J. Draper, University of Oxford; Alanine et al.26 N/A PfRH5DNL Simon J. Draper, University of Oxford; Wright et al.20 N/A Basigin Simon J. Draper, University of Oxford; Wright et al.20 N/A Critical commercial assays Amine Coupling Kit Sulfo-N-hydroxysuccinimide Cytiva Cat# BR1000-50 Human Pan-B cell enrichment kit StemCell Technologies Cat# 19554 ExpiFectamine 293 Transfection Kit ThermoFisher Scientific Cat# A14525 PCR purification kit Qiagen Cat# 28006 QIAprep spin miniprep kit Qiagen Cat# 27104 Deposited data Crystal structure of RH5DNL:R5.251 complex This paper PDB: 8QKR Crystal structure of RH5DNL:R5.034 complex This paper PDB: 8QKS Experimental models: Cell lines Human HEK293F ThermoFisher Scientific Cat# A14527 (Continued on next page) ll OPEN ACCESS Cell 187, 1–17.e1–e10, September 5, 2024 e2 Please cite this article in press as: Barrett et al., Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.015 Article .. Long, NIAID N/A P. falciparum NF54 Brandon K. Wilder, OHSU N/A Liver-humanized FRGN KO mice Yecuris N/A Oligonucleotides Primers for Vg, Vk, and Vl antibody heavy and light chain sequence reverse-transcription and nested PCR Hedda Wardemann; Tiller et al.48 N/A Primers for amplifying and cloning human Fab fragment sequences.
Purification:Article Title: Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype.
Article Snippet: Cat# NC15-Research Chemicals, peptides, and recombinant proteins Fetal Bovine Serum Biosera Cat# 60923 Penicillin streptomycin solution MilliporeSIgma Cat# P0781 L-glutamine MilliporeSigma Cat# G7513 RPMI 1640 Medium MilliporeSigma Cat# R0993 Viability dye FVS780 BD Biosciences Cat# 565388 Streptavidin-PE Invitrogen Cat# S866 Streptavidin-APC eBioscience Cat# 405207 Trizma hydrochloride solution (Tris-HCl) Sigma-Aldrich Cat# T3038 (Continued on next page) e1 Cell 187, 1–17.e1–e10, September 5, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNAsin Promega Cat# N2515 BshTI ThermoFisher Scientific Cat# FD1464 SalI ThermoFisher Scientific Cat# FD0644 XhoI ThermoFisher Scientific Cat# FD0694 Pfl23II ThermoFisher Scientific Cat# FD0854 LB agar plates with Amp-50 Teknova Cat# L1150 Dimethyl sulfoxide Sigma-Aldrich Cat# 20139 EX-CELL 420 medium Sigma-Aldrich Cat# 14420C Expi293 Expression medium ThermoFisher Scientific Cat# A1435101 Opti-MEM reduced serum medium ThermoFisher Scientific Cat# 31985070 Protein A Sepharose Fast Flow MilliporeSigma Cat# P3296 Phosphate buffered saline tablets Sigma-Aldrich Cat# P4417 CaptureSelect affinity C-Tag XL Affinity Matrix ThermoFisher Scientific Cat# 2943072010 Tween 20 Sigma-Aldrich Cat# P1379 Blocker Casein (thermos Fisher Scientific) ThermoFisher Scientific Cat# 37528 SIGMAFAST p-Nitrophenyl phosphate alkaline phosphatase substrate ThermoFisher Scientific Cat# 37620 Diethanolamine ThermoFisher Scientific Cat# 34064 Pierce Streptavidin coated plates ThermoFisher Scientific Cat# 15124 62 Biotinylated 20-mer PfRH5FL peptides Synthesised by Mimotopes, obtained from Simon J. Draper, Oxford University; Payne et al.18 N/A Ethanolamine (pH 8.5) Carterra Cat# 3626 Glycine (pH 2.0) Carterra Cat# 3640 Sodium acetate (pH 4.5) Carterra Cat# 3623 MES Carterra Cat# 3625 HEPES Sigma-Aldrich Cat# H3375 Hypoxanthine Sigma-Aldrich Cat# H9377 HiTrap Protein G column Cytiva Cat# 17040501 LambdaFabSelect column Cytiva Cat# 17548211 RH5.1 Simon J. Draper, University of Oxford; Jin et al.16 N/A PfRH5DNC-Biotin This paper N/A PfCyRPA Simon J. Draper, University of Oxford; Alanine et al.26 N/A PfRH5DNL Simon J. Draper, University of Oxford; Wright et al.20 N/A Basigin Simon J. Draper, University of Oxford; Wright et al.20 N/A Critical commercial assays Amine Coupling Kit Sulfo-N-hydroxysuccinimide Cytiva Cat# BR1000-50 Human Pan-B cell enrichment kit StemCell Technologies Cat# 19554 ExpiFectamine 293 Transfection Kit ThermoFisher Scientific Cat# A14525 PCR purification kit Qiagen Cat# 28006 QIAprep spin miniprep kit Qiagen Cat# 27104 Deposited data Crystal structure of RH5DNL:R5.251 complex This paper PDB: 8QKR Crystal structure of RH5DNL:R5.034 complex This paper PDB: 8QKS Experimental models: Cell lines Human HEK293F ThermoFisher Scientific Cat# A14527 (Continued on next page) ll OPEN ACCESS Cell 187, 1–17.e1–e10, September 5, 2024 e2 Please cite this article in press as: Barrett et al., Analysis of the diverse antigenic landscape of the malaria protein RH5 identifies a potent vaccine-induced human public antibody clonotype, Cell (2024), https://doi.org/10.1016/j.cell.2024.06.015 Article .. Long, NIAID N/A P. falciparum NF54 Brandon K. Wilder, OHSU N/A Liver-humanized FRGN KO mice Yecuris N/A Oligonucleotides Primers for Vg, Vk, and Vl antibody heavy and light chain sequence reverse-transcription and nested PCR Hedda Wardemann; Tiller et al.48 N/A Primers for amplifying and cloning human Fab fragment sequences.
|
Teknova is a verified supplier 
Teknova manufactures this product 